OrpeGen is a biotechnology company specializing in the production and distribution of high-quality antibodies for research and diagnostic purposes. They offer a wide range of monoclonal and polyclonal antibodies, as well as custom antibody development services. Their products are used in various fields including immunology, oncology, and infectious diseases.
OrpeGen also focuses on providing innovative solutions to meet the specific needs of researchers and clinicians. Their offerings include antibodies for flow cytometry, ELISA, western blotting, and immunohistochemistry, ensuring comprehensive support for different experimental and diagnostic approaches. The company is dedicated to maintaining high standards in the production of antibodies, emphasizing both quality and reproducibility.
NEWS
BASOTEST
BASOTEST allows the quantitative determination of human basophil degranulation in heparinized whole blood. It contains the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP) as stimulant, a two-colour antibody reagent for detection of basophilic granulocytes and their activation and necessary reagents. It determines the percentage of basophilic granulocytes which have degranulated after incubation with allergen or fMLP.
APPLICATIONS
BASOTEST is intended to investigate allergen induced activation of basophilic granulocytes.
Basophilic granulocytes are the least common circulating leucocyte in blood and account for only 0.5-1% of the total white blood cell population. Immediate-type hypersensitivity is the hallmark of any allergic reaction. Basophils have high affinity Fc receptors for IgE antibodies on their surface. Allergic reactions are predominantly due to the immunoglobulin E (IgE) class of antibodies and develop when contact with a specific antigen triggers the formation of IgE antibodies which bind to the Fc receptors of basophilic leukocytes. Renewed contact with the same antigen then leads to bridging: adjacent antibodies on the cell surface are interconnected by antigen molecules through their antigen-binding portions. This bridging of IgE molecules on the basophil cell surface by antigen or allergen activates the cell to secrete its mediators. A number of chemical mediators are released from basophils. The secretory granules contain a preformed mediators, such as histamin, heparin, neutral protease, and a number of acid hydrolases and chemotactic factors. Secondary mediators (leukotrienes) are also generated as a result of cell activation. Allergic rhinitis, bronchial asthma and urticariia are typical allergic diseases.
The flow cytometric method correlates very well with histamine release. BASOTEST therefore allows the diagnosis of immediate-type hypersensitivity.
PRINCIPLES
BASOTEST allows the quantitative determination of human basophil activation and degranulation.
The test kit contains the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP) as positive control, a two-colour antibody reagent for detection of basophilic granulocytes and determination of basophil activation and necessary reagents. Heparinized whole blood is incubated with allergen at various concentrations for 30 min at 37°C. The chemotactic peptide N-Formyl-Met-Leu-Phe (fMLP) is used as a positive control at a concentration of 1 µM, addition of WASHING SOLUTION serves as negative background control. Activation of basophilic granulocytes induces fusion of cytoplasmic granules with the plasma membrane and the sucessive release of inflammatory mediators. After incubation at 37°C the cell suspension is washed once with WASHING SOLUTION. The cells are then stained with the two-colour antibody reagent consisting of two different murine monoclonal antibodies conjugated with various fluorochromes. The monoclonal antibody anti-IgE-PE is conjugated with the fluorescent dye Phycoerythrin, reacts with human IgE and therefore detects basophilic granulocytes. The monoclonal antibody anti-gp53-FITC is conjugated with the fluorochrome fluorescein and recognizes a glycoprotein (gp53), which is expressed on activated basophils. After staining of basophils with this antibody reagent the cells are washed with WASHING SOLUTION, erythrocytes are removed by addition of LYSING SOLUTION. After one additional washing step, the percentage of activated basophilic granulocytes is determined by flow cytometry.
REAGENTS PROVIDED
- STAINING REAGENT (anti-IgE-PE and anti-gp53-FITC)
- fMLP
- LYSING SOLUTION
- WASHING SOLUTION
ORPEGEN Pharma GmbH offers a broad spectrum of products and services, including:
Production of diagnostic test kits for flow cytometry and fluorescence microscopy
Development and biotechnical production of recombinant proteins by cell culture
Large scale production of amino acid derivates from kg to tons (tert. butylesters & ethers, Fmoc-, Boc-, Ddz-, Z-)
Production and development of pharmaceutical peptides from mg to kg based on GMP standards and PIC (WHO) approval (antigenic determinants, hormones, immunoregulatory fragments etc.)
Contract research and development on molecular biology, cell biology, immunology, peptide and protein chemistry .
Development and Biotechnological
Biotechnological Production
Development and Biotechnological Production of Recombinant Proteins
Grafik Proteins for therapy and diagnostics from natural or recombinant organisms under GMP (Good Manufacturing Practice) and PIC (Pharmaceutical Inspection Convention)
Biotechnological production and downstream purification of proteins from mammalian and other eucaryotic cells (monoclonal antibodies, glycoproteins, etc.)
Large scale cell culture (roller bottle cultivation, hollow fiber and membrane systems, fluidized bed reactor)
In house analysis of cells and product by the departments of molecular biology(e.g., copy number, contaminant DNA), cell biology (e.g., identity, stability, biological activity), immunology (e.g., activity, contaminant proteins), cytometry (biological activity, immune response) and protein chemistry (e.g., purity, identity) Production of Recombinant Proteins
Services proposés
Notre expertise
Projets innovants
Des images de haute qualité
ORPEGEN Pharma GmbH offers a broad spectrum of products and services, including:
- diagnostic test kits for flow cytometry
- biotechnical production of recombinant proteins by cell culture
- Large scale production of amino acid derivates
- pharmaceutical peptides from mg to kg based on GMP standards and PIC (WHO) approval
- Contract research and development on molecular biology, cell biology, immunology, peptide and protein chemistry
Quantitative determination of the chemotactic function of neutrophilic granulocytes
Product of ORPEGEN Pharma
Simple and sensitive method for the flow cytometric determination of the chemotactic function of neutrophilic granulocytes.
Test kit contains multiwell plates plates and precoated cell culture inserts for chemotaxis studies, the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP), an antibody reagent, counting beads and necessary reagents.
Biotechnological Production
Development and Biotechnological Production of Recombinant Proteins
Grafik Proteins for therapy and diagnostics from natural or recombinant organisms under GMP (Good Manufacturing Practice) and PIC (Pharmaceutical Inspection Convention)
Biotechnological production and downstream purification of proteins from mammalian and other eucaryotic cells (monoclonal antibodies, glycoproteins, etc.)
Large scale cell culture (roller bottle cultivation, hollow fiber and membrane systems, fluidized bed reactor)
In house analysis of cells and product by the departments of molecular biology(e.g., copy number, contaminant DNA), cell biology (e.g., identity, stability, biological activity), immunology (e.g., activity, contaminant proteins), cytometry (biological activity, immune response) and protein chemistry (e.g., purity, identity)
Grafik Proteins for therapy and diagnostics from natural or recombinant organisms under GMP (Good Manufacturing Practice) and PIC (Pharmaceutical Inspection Convention)
Biotechnological production and downstream purification of proteins from mammalian and other eucaryotic cells (monoclonal antibodies, glycoproteins, etc.)
Large scale cell culture (roller bottle cultivation, hollow fiber and membrane systems, fluidized bed reactor)
In house analysis of cells and product by the departments of molecular biology(e.g., copy number, contaminant DNA), cell biology (e.g., identity, stability, biological activity), immunology (e.g., activity, contaminant proteins), cytometry (biological activity, immune response) and protein chemistry (e.g., purity, identity)
Clinical Diagnostic for the Determination of Allergen Induced Basophil Degranulation
Product of ORPEGEN Pharma
Simple:
Whole blood assay for clinical routine analysis. Does not require isolation procedures
Fast:
Data within 2 hours.
Sensitive:
Stimulation buffer amplifies the signal.
Diagnostic:
Standardized test procedure for - detecting immediate-type hypersensitivity - testing of natural allergens - checking the success of immunotherapy (e.g. hyposensitisation)
Proven:
Good correlation with histamine release assay.
Complete:
Kit contains important allergens plus positive and negative controls. No disturbance by potential histamine in allergen extracts.
Large Scale Production of Amino Acid Derivatives from kg to tons
Contract Research
Contract Research and Development
ORPEGEN Pharma undertakes contract research and development
on peptides and proteins: synthesis, sequencing, HPLC protocols, protein characterization by SDS-PAGE, Western blot, IEF, carbohydrate analysis, and enzymatic mapping
in biotechnology: pharmaceutical grade proteins from natural sources, proteins from recombinant mammalian cell cultures, establishment of recombinant cell lines, purification and quality control, process validation
in molecular biology: quantitation of host cell derived DNA determination (contaminant DNA assay, Threshold -system), DNA amplification, cloning, sequencing, construction of expression vectors, investigation of the stability of production cell lines by pulsed field gel electrophoresis (PFGE), detection of mutations by temperature gradient gel electrophoresis (TGGE), primers and probes, viral decontaminations.
in cell biology and immunology: contract production of monoclonal antibodies (roller bottle cultivation/bioreactor from Endotronics, AcuSyst Junior), in vitro assays for the screening of immunopharmacologically active compounds (flow cytometric analysis using PHAGOTEST®, BURSTTEST (PHAGOBURST) and NKTEST, cytokine release, receptor activation), in vivo models, sensitive contaminant or residual protein assay based on ILA (Threshold system), preclinical studies and clinical monitoring by flow cytometry (FACScan), development and validation of ELISA systems, LAL-test. Bioassays: Evaluation of the bioactivity of human growth hormone (hGH) and of lactogenic hormones such as prolactin (Prl) using the Nb2 cell bioassax, other cell line-dependent bioassays.